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Image Search Results
Journal: mBio
Article Title: Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models
doi: 10.1128/mbio.00184-24
Figure Lengend Snippet: Species composition of biofilms grown on hydroxyapatite discs with feast/famine cycles. (A) Schematic of media changes for the feast-and-famine dental biofilm model. (B) Final counts of CFUs for each microbial species at the end of the 5-day experiment. CFUs for biofilms grown in the presence of 0.2 mM NaF are normalized relative to biofilms grown in the presence of 0.2 mM NaCl. Unnormalized counts from the fluoride-treated and control biofilms are shown in Fig. S3. (C) S. mutans CFUs recovered from biofilms of the indicated composition after each treatment in the feast and famine cycle. Statistical significance is shown relative to all-WT fluoride-treated biofilms (open circles). (D). C. albicans CFUs recovered from biofilms of the indicated composition after each treatment in the feast and famine cycle. Statistical significance is shown relative to all-WT biofilms and fluoride-treated biofilms (open circles). (E) S. gordonii CFUs recovered from biofilms of the indicated composition after each treatment in the feast-and-famine cycle. Statistical significance is shown relative to all-WT biofilms and fluoride-treated biofilms (open circles). For all panels, datapoints represent the mean and SEM of three independent experiments ( n = 3). Significance was calculated using a two-way analysis of variance (ANOVA) followed by Fisher’s LSD test. Statistical significance is shown relative to parallel experiments in the presence of 0.2 mm NaCl and is represented as *p < 0.05, **p < 0.01, and *** p < 0.001.
Article Snippet:
Techniques: Control
Journal: mBio
Article Title: Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models
doi: 10.1128/mbio.00184-24
Figure Lengend Snippet: Properties of biofilms grown on hydroxyapatite discs with feast/famine cycles. (A) Media pH was measured after each cycle of feast and famine. (B) Calcium concentration in media was measured after each cycle of feast and famine. For panels A and B, for each biofilm composition, control experiments were performed in the presence of NaCl (black points/lines in panels A and B). These show close correspondence with overlapping points. For the fluoride-treated conditions, statistical significance is shown relative to the chloride-treated biofilms and is represented as * p < 0.05, ** p < 0.01, and *** p < 0.001. (C) Final biofilm mass at the end of the 5-day experiment. (D). Fluoride content of biofilm at the end of the 5-day experiment. For all panels, datapoints represent the mean and SEM of three independent experiments ( n = 3). Significance was calculated using a two-way analysis of variance (ANOVA) followed by Fisher’s LSD test.
Article Snippet:
Techniques: Concentration Assay, Control